Red oil was prepared by extraction of the plant material with ether, followed by distillation of the concentrated extract at room pressure followed by redistillation under reduced pressure (15-50 mm Hg). The oil was acetylated with acetic anhydride, and the acetylated product was subjected to fractional distillation in vacuo. Six fractions were collected. The head and tail fractions were removed. The remaining four fractions which represented the principal fractions (fractions 2, 3, 4, and 5) were combined and passed over silica gel column in benzene and then passed over activated alumina in carbon tetrachloride solution. The product was hydrolyzed by acid, alkali, or ammonia in alcoholic solution. The authors reported that the deacetylated product has, in each case, a different physiological potency than the acetate. All fractions were not pure compounds.
DeRopp, in 1960 (12), described the isolation of THC from the flowering tops of Cannabis sativa. His method involved adsorption chromatography of the methanolic extract of cannabis followed by partition chromatography on Celite using N,N-dimethyl formamide/cychlohexane mixture and high vacuum distillation. The purity of THC was based on paper chromatographic evidence.
The first isolation of the naturally occurring THC in its pure form was reported by Gaoni and Mechoulam in 1964 (13). THC was isolated from the hexane extract of hashish by repeated column chromatography on florisil and alumina. Further purification was carried out by the preparation of the crystalline 3,5-dinitrophenylurethane of THC followed by mild basic hydrolysis to get the pure THC. The purity of THC was proven by thin layer chromatography (TLC) and spectroscopic analysis (IR and NMR).
Korte, et al., in 1965 (14) reported the isolation of THC from the crude extracts of the female inflorescence of Cannabis sativa indica and Cannabis sativa non indica. The crude extracts were chromatographed over activated alumina in order to remove the coloring impurities like carotinoids, chlorophylls, and xanthophylls. All the cannabinolic fractions were combined and concentrated to give a brownish-red oil. The oil was further purified by a countercurrent distribution method to get THC which was proved to be identical with that described by Gaoni and Mechoulam (13).
